Détails de la réservation
- Le : 14 September 2022
- De : 10 h 00
- à : 12 h 30
- Salle : Salle de conférence
Détails de l'évènement
Prof Yorifumi Satou
Division de génomique et transcriptomique du Joint Research Center
for Human Retrovirus Infection (Kumamoto University, Japon)
"Single cell analysis to elucidate mechanisms underlying oncogenesis caused by HTLV-1"
Abstract :
Human T-cell leukemia virus type 1 (HTLV-1) mainly infect CD4+ T-cells and induces chronic, persistent
infection in infected individuals with some progressing to develop adult T-cell leukemia/lymphoma
(ATL). HTLV-1 alters cellular differentiation, activation and survival but it is unknown if this contributes to
malignant transformation. In this study, we used single-cell RNA-sequencing and TCR-sequencing to
investigate T-cell differentiation and HTLV-1-mediated transformation processes. We analyzed 87,742
single cells from peripheral blood of 12 infected and 3 uninfected individuals. Trajectory analysis
successfully captured physiological T-cell activation dynamics as well as ATL progression as a continuous
process. Naive T-cells dynamically change into activated T-cells including infected cells, which
seamlessly transitioned into ATL cells characterized by clonally expanded, highly-activated T-cells. In
conclusion, our study revealed the mechanisms of HTLV-1-mediated transformation in vivo at single-cell
level. (Tan BJY et al, JCI, 131(24):e150472, 2021)
Responsable
- Nom : Paul Lesbats