Détails de la réservation
- Le : 14 December 2016
- De : 12 h 30
- à : 17 h 30
- Salle : Salle de conférence
Détails de l'évènement
Thèse Xiaomin ZHANG Ph.D student / Interdisciplinary Institute for NeuroScience, Bordeaux / Max Planck Institute of Experimental Medicine, Göttingen "Axonal homeostasis of VGLUT1 synaptic vesicles in mice"
Synaptic vesicles (SVs) are essential for neurotransmission, and more efforts are needed to better understand their neurotransmitter content, release kinetics, distribution and mobility. SVs are not only clustered in the presynaptic boutons, but also dynamically shared among multiple en passant presynaptic boutons, a phenomenon named SV super-pool. Previous work from our laboratory suggested that Vesicular GLUtamate Transporter 1 (VGLUT1) may play a role in regulating SV super-pool size beyond loading glutamate into SV. My Ph.D project is focused on SVs mobility in axons.
Firstly, I generated a VGLUT1mEos2 knock-in (KI) mouse line, which provides extended possibilities to study SV trafficking and characterize SV super-pool. Secondly, I engaged in a thorough VGLUT1 structure-function analysis. I identified that VGLUT1 tends to cluster SVs in the presynaptic boutons and reduce SVs exchange with the super-pool via the second poly-proline motif of its C-terminus. Overall, my Ph.D work contributes to the knowledge of the role of VGLUT1 in regulating SVs mobility and provides new tools for the further investigations on SV super-pool physiology.
Keywords : synaptic vesicle mobility, VGLUT1, super-pool, live imaging, structure-function analyses.
Responsable
- Nom : Xiaomin Zhang