Détails de la réservation
- Le : 27 November 2017
- De : 13 h 30
- à : 18 h 00
- Salle : Amphi Centre Broca Nouvelle - Aquitaine
Détails de l'évènement
Matthieu Lagardère
"Development of a Brownian Dynamics Simulation Software dedicated to Live Cell Fluorescence Imaging"
Résumé
Fluorescence has revolutionized cellular imaging: by deeply increasing contrast, it permits to image cellular structures, and to follow molecular dynamics, which were not achievable by other optical imaging methods. Nowadays, a great number of optical microscopy techniques use the properties of fluorescent dyes and proteins. They have allowed scientists to observe finer and finer structures, to study the dynamics of an ensemble of molecules (FRAP, PAF) or of individual particles (SPT, FCS) but also to investigate the interactions between molecules in live conditions (FCCS, FRET). However, these experimental paradigms are complex and numerous biases can introduce inaccuracy in the obtained results.
To the aim of precisely interpreting live cell fluorescence imaging experiments, we have developed a simulation software which takes into account the characteristic parameters of these experiments. This software, which provides a graphical interface to adjust in real time the simulation parameters, utilizes Monte Carlo Methods to simulate the Brownian motion of single molecules and the photophysics of associated fluorophores. These molecules diffuse in a user-defined geometry and can transit between fast and slow diffusion (trapping state), depending on the cellular compartment. Fluorophores can be fluorescent, blinked or photobleached. Transition rates between these states are used to simulate the fluorescence experiments.
We show that the simulations generated by the algorithm are in accordance with the theoretical results typically used to analyze fluorescence experiment outputs. Then, we use the software to study an artificial cell-cell junction made up with two COS cells overlapping with each other. This junction, called adhesive contact, is mediated by two adhesion proteins called neurexin and neuroligin which establish a heterologous complex. Fluorescence imaging experiments (SPT, FRAP, FCS) have been performed, and are interpreted using simulations generated by our software. In this study, we show that the dynamic properties of neurexin are modified in the cell-cell contact area in accordance with the model.
Responsable
- Nom : Lagardère Matthieu